A note on serum nucleotidase determinations.
نویسندگان
چکیده
Current methods for estimating 5'-nucleotidase activity in human serum are critically discussed. The requirements for specificity in the assay procedure are re-emphasised, special consideration being given to the absolute concentrations as well as the molar ratios of true and alternative substrates. Substrate-inhibition, described in a previous Report is believed to be due to a non-linear relationship between AMP concentration and optical density above 0.1 mM.
منابع مشابه
Normal ranges and diagnostic value of serum 5'nucleotidase and alkaline phosphatase activities in infancy.
Belfield, A., and Goldberg, D. M. (1971). Archives of Disease in Childhood, 46, 842. Normal ranges and diagnostic value of serum 5'nucleotidase and alkaline phosphatase activities in infancy. Determinations of serum alkaline phosphatase (APase) and 5'nucleotidase (5Nase) activities have been carried out in 159 children investigated for possible disease of liver or bones, and in 147 children in ...
متن کاملThe clinical biochemistry of 5'-nucleotidase.
This review delineates the subcellular distribution, biochemical characteristics, and metabolic functions of 5'-nucleotidase (5'NT), summarizes the analytical biochemistry of 5'NT, and assesses the clinical significance of 5'NT determinations in body fluids, cells, and tissues. Salient aspects of the clinical biochemistry of 5'NT, discussed herein, are as follows: (A) Serum 5'NT activity is gen...
متن کاملInhibition by concanavalin A as the basis for a specific assay of serum 5'-nucleotidase activity.
Concanavalin A inhibits serum 5'-nucleotidase activity, without causing significant inhibition of alkaline phosphatase activity. This observation serves as the basis for a new method for assaying the 5'-nucleotidase activity in serum, which depends upon the difference between the enzymic hydrolysis of adenosine-5'-monophosphate in the presence and absence of concanavalin A. A denosine released ...
متن کاملBiological variance of cholinesterase and 5'-nucleotidase in serum of healthy persons.
We measured cholinesterase (EC 3.1.1.8) and 5'-nucleotidase (EC 3.1.3.5) activities in serum of 24 healthy laboratory staff during 12 months. Overall mean activities ranged from 5.3 to 13.4 kU/L for cholinesterase and 5.4 to 9.8 U/L for 5'-nucleotidase. Cholinesterase activity was significantly (p less than 0.01) higher for men than for women. 5'-Nucleotidase activity was significantly (p = 0.0...
متن کاملSuppressive effect of culture supernatant of erythrocytes and serum from dogs infected with Babesia gibsoni on the morphological maturation of canine reticulocytes in vitro
The present study evaluated the effects of infected culture supernatant of erythrocytes, fractionation of culture supernatant and serum from dogs infected with Babesia gibsoni (B. gibsoni) on the maturation of canine reticulocytes in vitro. The SDS-PAGE demonstrated that significantly broader bands were generated by both the infected culture supernatant of erythrocytes and the serum from dogs c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Zeitschrift fur klinische Chemie und klinische Biochemie
دوره 9 3 شماره
صفحات -
تاریخ انتشار 1971